Glutamate transport into synaptic vesicles. Roles of membrane potential, pH gradient, and intravesicular pH.
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چکیده
منابع مشابه
Sodium gradient-dependent L-glutamate transport in renal brush border membrane vesicles. Effect of an intravesicular > extravesicular potassium gradient.
A Na+ gradient (extravesicular > intravesicular) increased approximately 10 times the initial rate of Lglutamate transport into osmotically active renal brush border membrane vesicles and energized the transient accumulation of the amino acid against its concentration gradient. Stimulation of glutamate uptake was specific for Na'. Increasing the concentration of extravesicular Na' increased the...
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The transport mechanism of urate and p-aminohippurate (PAH) was evaluated in microvillus membrane vesicles isolated from the renal cortex of the mongrel dog. Imposition of a transmembrane pH gradient (pHo less than pH1) markedly accelerated the uptake of [14C]urate and [3H]PAH and caused the transient accumulation ("overshoot") of each anion above its final level of uptake. The transport of ura...
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The regulation of lysosomal cystine transport was studied using cystine dimethyl ester-loaded lysosomes isolated from human diploid fibroblasts. Net efflux from normal fibroblast lysosomes was compared to that from lysosomes of cystinotic fibroblasts, which contain an inherited mutation decreasing lysosomal cystine transport. This exodus of cystine from normal fibroblast lysosomes was greater t...
متن کاملCharacterization of glutamate uptake into synaptic vesicles.
Recent evidence indicates that L-glutamate is taken up into synaptic vesicles in an ATP-dependent manner, supporting the notion that synaptic vesicles may be involved in glutamate synaptic transmission. In this study, we further characterized the ATP-dependent vesicular uptake of glutamate. Evidence is provided that a Mg-ATPase, not Ca-ATPase, is responsible for the ATP hydrolysis coupled to th...
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Electrochemical proton gradients are the basis of energy transduction in modern cells, and may have played important roles in even the earliest cell-like structures. We have investigated the conditions under which pH gradients are maintained across the membranes of fatty acid vesicles, a model of early cell membranes. We show that pH gradients across such membranes decay rapidly in the presence...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)49549-5